Degradation insight of organophosphate pesticide chlorpyrifos through novel intermediate 2,6-dihydroxypyridine by Arthrobacter sp. HM01

Abstract Organophosphates (OPs) are hazardous pesticides, but an indispensable part of modern agriculture; collaterally contaminating agricultural soil and surrounding water. They have raised serious food safety environmental toxicity that adversely affect the terrestrial aquatic ecosystems therefore, it become essential to develop a rapid bioremediation technique for restoring pristine environment. A newly OPs degrading Arthrobacter sp. HM01 was isolated from pesticide-contaminated identified by ribotyping (16S rRNA) method. Genus has not been previously reported in chlorpyrifos (CP) degradation, which shows 99% CP (100 mg L −1 ) degradation within 10 h mMSM medium also tolerance high concentration (1000 CP. utilized broad range pesticides other aromatic pollutants including intermediates as sole carbon sources. The maximum obtained at pH 7 32 °C. During intermediate 2,6-dihydroxypyridine detected through TLC/HPLC/LCMS analysis putative pathway proposed its degradation. study revealed organophosphate hydrolase ( opd H) gene responsible opdH-enzyme located intracellularly. opdH enzyme characterized cell free extract optimum temperature requirement, 7.0 50 °C, respectively. Thus, results true potential OPs-bioremediation. Moreover, strain showed fastest rate among Graphical